18.4 Agricultural mycocides at the start of the 21st century: strobilurins

Azoles are important agricultural fungicides and we have indicated above some of their uses (Fig. 5 above). Although the search for new azole fungicides is an active research area, particularly for the more damaging plant pathogens such as the causal agent of rice blast disease, Magnaporthe grisea (Mares et al., 2006), use of clinically useful antimicrobials in agriculture reduces their value in the clinical setting because broadcast application (for example by aerial spraying or dusting of crops in the field) exposes enormous populations of microbes to selective pressure for antibiotic resistance.

This is a particular problem for antibacterials where horizontal transfer of plasmids carrying resistance can communicate field acquired resistance to commensals and pathogens in the human population. The problem is no less acute in fungi. Horizontal transfer of resistance on plasmids does not occur but so many of the most troublesome opportunistic fungal pathogens are common soil and environmental fungi that field acquired resistance can still easily enter the human population.

There are alternatives to azoles:

  • Melanised walls are important to the survival and functioning of many plant pathogens and the azole fungicide tricyclazole (Fig. 11) inhibits melanin biosynthesis and is effective against fungal plant pathogens that require a melanised appressoria to penetrate the plant cell wall (e.g. Botrytis & Magnaporthe).
  • use of nikkomycin and polyoxorim (polyoxin-D) as fungicides that act as competitive inhibitors of chitin synthase is illustrated in Fig. 8;
  • mitotic inhibitors benomyl and giseofulvin are illustrated in Fig. 10;
  • edifenphos (Fig. 11) is an organophosphorus ester that is used as a fungicide in agriculture mainly to control rice diseases. Edifenphos is moderately toxic to man, being rapidly absorbed and able to cause liver, kidney and nerve damage.
  • Metalaxyl (Fig. 11), an acylalanine fungicide, inhibits RNA polymerase I in Oomycota (Kingdom Chromista) such as the cause of potato late blight, Phytophthora infestans. Resistance to this fungicide commonly arises.
Structural formulae of the fungicides edifenphos, metalaxyl, and tricyclazole
Fig. 11. Structural formulae of the fungicides edifenphos (an organophosphorus ester), metalaxyl (an acylalanine fungicide, the (-)-isomer is illustrated here), and the melanin-synthesis inhibitor, tricyclazole.

Among the most widely used and most effective classes of agricultural fungicides in the world today are a class of chemicals called strobilurins, which were first discovered in 1977, although the first useful products were not on sale until 1996. These are broad-spectrum fungicides, effective against a wide range of of fungal pathogens and are a relatively recent addition to the market. Strobilurins work by inhibiting mitochondrial respiration by blocking electron transport. This means that the fungus cannot produce energy so can no longer grow and eventually dies. Because Strobilurins are derived from a natural product, they are considered to be environmentally safe because they are rapidly degraded.

The active ingredients of strobilurin fungicides are synthetic analogues of natural secondary metabolites known as p-methoxyacrylic acids, specifically: strobilurin A, oudemansin A, and myxothiazol A (Fig. 12).

The original (natural) strobilurins

Fig. 12. The original (natural) strobilurins. Note the characteristic chemical structures: methoxy (CH3-O-) and acrylate (CH2=CHCOO−, which features a vinyl group, that is, two carbon atoms double bonded to each other and directly attached to a carbonyl carbon).

Strobilurin compounds were discovered in the basidiomycete mushroom fungi Oudemansiella mucida, Pseudohiatula esculenta var. tenacellus (a synonym for Strobilurus stephanocystis) and Strobiluris tenacellus. It is counterintuitive to expect fungi to produce fungicides; as Balba (2007) puts it: '...Many scientists excluded the idea of looking for fungicides in fungus...(sic)'

However, once Strobilurin A had been isolated from liquid cultures of the mushroom Strobilurus tenacellus the fungicides were found in culture filtrates of many other fungi, and with the benefit of hindsight we can understand why fungicides make a contribution to the biology of the producing organism by contributing to its competitive ability.

Strobilurus tenacellus is a saprotrophic wood rotting fungus which characteristically grows and fruits on the pine cones of Pinus sylvestris. The production of strobilurins enables S. tenacellus to predominate in these small substrata despite potential competition from arthropods and other fungi. Strobilurin acts as a feeding deterrent to insects that might compete for the substrate and/or graze the S. tenacellus mycelium. More importantly, strobilurins are fungicidal to all the common fungi that might compete for the resource, whether they be Ascomycota, Basidiomycota or Oomycota.

This activity identified strobilurins as possible commercial antifungal agents; they are effective against all major classes of pathogenic fungi and fungus-like organisms;

  • Ascomycota like powdery mildew and rice blast,
  • Basidiomycota like rusts,
  • Oomycota such as downy mildews.

In 1992 the first artificial strobilurin fungicide was announced and was on the market in 1996 (Sauter, Steglich & Anke, 1999; Bartlett et al., 2002; and see our Resources Box A Short History of Fungicides). Strobilurins were so successful that by 1999 they accounted for 10% of the global fungicide market, reaching 15% by 2005. As a result of their widespread use on cereals and soybeans, strobilurins are now the second largest group of fungicidal chemicals in use in agriculture, being second only to the triazoles. So within ten years of their introduction to the market the stobilurins had surpassed all of the older fungicide groups in importance excepting only for the triazoles.

Resources Box

A Short History of Fungicides

by Vince Morton & Theodor Staub (both formerly of Novartis Crop Protection, now Syngenta).

This is an APSnet Feature dated March 2008 (APS = American Phytopathological Society).

CLICK HERE to download as a PDF or visit the original website at this URL: http://www.apsnet.org/online/feature/fungi/


Strobilurins inhibit respiration at a single unique site that blocks ATP synthesis (see below). This single site specificity raises the problem of potential resistance. Of course, the producing organisms are resistant to their own strobilurins, which itself illustrates that fungi can mutate to strobilurin resistance. Several resistant fungal pathogens have been identified in the field, and fungicide resistance affects Septoria diseases of wheat in Europe, and of turf in the US. In response, fungicide production companies are continually researching new strobilurins, developing mixtures with fungicides that have other active principles, and developing new methods of application, such as seed treatments.

Strobilurins act by blocking oxidative phosphorylation in the fungal mitochondrial membrane. The standard pathway for NADH oxidation in mitochondrial respiration involves three protein complexes, I, III and IV. These complexes are involved in energy conservation by linking NADH oxidation and electron/proton translocation (ten protons per two electrons) with ATP synthesis.  

Strobilurins bind to the ubiquinone (= coenzyme Q) carrier that carries electrons to the cytochrome b-c1 complex (described in the section The endomembrane systems of Chapter 5; CLICK HERE to view the page, or CLICK HERE to view the Virtual Cell Animation describing the Mitochondrial Electron Transport Chain).

Fungi that produce strobilurins have a modified amino acid sequence in the binding envelope of the coenzyme Q protein that greatly reduces its binding affinity for strobilurin, making the strobilurin-producer strobilurin-resistant (Sauter et al., 1999).

The mode of action of the strobilurins is unique to them. It has made them an important aid in research aimed at understanding the details of mitochondrial respiration, but it was also promptly recognised as commercially unique; the significance being that resistance to other established fungicides would not confer resistance to strobilurin.

The natural products themselves are not suitable for agricultural use because they are volatile and unstable in the light. Many agrochemical companies invested in research to produce synthetic analogues of strobilurins, and related compounds (predominantly of strobilurin A due to its simpler structure for chemical synthesis), and a race to patent the first strobilurin fungicide ensued (Sauter et al., 1999; Bartlett et al., 2002) (Table 8 shows available strobilurin fungicides and their dates of release).

Table 8. The strobilurin fungicides       




First sales

























*Structural formulae are shown in Fig. 13. Azoxystrobin was discovered by ICI, the agrochemical interests of which are now part of Syngenta. Trifloxystrobin was discovered by Novartis and sold to Bayer in 2000. Data from Bartlett et al., 2002.


Structural formulae of commercial strobilurin fungicides

Fig. 13. Structural formulae of commercial strobilurin fungicides (refer to Table 8).

Chemical modification concentrates on improving photostability (diphenyl ethers improve stability) and reducing volatility (by adding benzene rings). Another concern is to improve the systemic properties of the strobilurins, which enhances performance and reduces application rates as a result of the active redistribution of systemic agents throughout the plant. There is considerable scope for variation in the strobilurin side chains, and this enables steady application of structural changes to improve product functionality (see Table 8 and Fig. 13).

The effectiveness of strobilurins is highly dependent on the timing of application, especially on the stage of growth and its respirational demands. Pyraclostrobin and trifloxystrobin are very effective against Cercospora beticola on sugar beet; spore germination is effectively inhibited and spore production by diseased leaves is significantly reduced, both of these effects are due to the high demand for ATP during spore germination and development. There is much less inhibition of hyphal growth; this is presumably due to hyphae having different, and perhaps alternative, respiration demands.

A commercial disadvantage of the mode of action of strobilurins is that their target site is in the universal pathway of respiration, yet the fungicides need to remain non-toxic to non-target organisms. Toxicity to animals varies between the different strobilurins, but strobilurins in general have been classified as having minimal risk to human health. Selectivity can be modified by manipulation of the chemical structure; and can be fine-tuned by control of dosage in the field. For example, the activity of Glomus coronatum mycorrhiza in five week old maize plants was unaffected by strobilurin treatment to the leaf surfaces at recommended commercial doses.

There is evidence that strobilurin treated plants show positive physiological side effects that result in increased crop yields. In cereals an increase in biomass due to elevated carbohydrate synthesis and consequent increase in photosynthetic capabilities has been observed. Similarly, strobilurin treatment to control early blight in potatoes (caused by Alternaria solani) resulted in increased yield, greater tuber size and improved crop value. Generally speaking, these yield improvements following strobilurin treatments are higher than can be explained only by their fungicidal activity, and the response is not seen when non-strobilurin fungicides, such as azoles, are used.

Development of resistance is an issue with all fungicides and the strobilurins are produced by fungi. Fungi that make the natural products have one of five single nucleotide substitutions in their cytochrome c gene that cause them to be unaffected by the compounds they produce. One of these substitutions is the causative mutation in many resistant pathogen mutants. However, the most damaging emerging resistance to strobilurins is associated with an alternative pathway that can bypass the strobilurin target site. Alternative oxidase (AOX) is a strobilurin-insensitive terminal oxidase which is present in many fungi. AOX accepts electrons directly from ubiquinol and acts as a substitute to the strobilurin target complex. Fungal AOX activity is inhibited by salicyl hydroxamic acid, but this also inhibits the plant AOX and is not a useful selective inhibitor.

Resistance to strobilurins does not confer cross resistance to fungicides with different modes of action, like the azoles. But there are two recently introduced fungicides, famoxadone (a dicarboximide/oxazole fungicide that first went on sale in 1997) and fenamidone (an imidazole fungicide released for sale in 2001) that have the same mode of action as strobilurins and are susceptible to strobilurin resistance.

There is a Fungicide Resistance Action Committee (FRAC; view http://www.frac.info/frac/index.htm) that recommends usage patterns and devises approaches that prolong the effectiveness of fungicides liable to encounter resistance problems and limits crop losses when resistance appears. The likelihood that resistance will arise can be minimised by restricting the number of applications per season so that no more than two sequential applications are made. Alternating to a non-strobilurin fungicide with a different mode of action is preferable to sequential application of strobilurin. FRAC recommendations need to be strictly maintained to sustain the high efficacy of this class of fungicide and limit the impact of resistance on strobilurin performance.

Updated December 17, 2016