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Fungiflex: the untold storybyDavid Moore & LilyAnn Novak FrazerIn this publication, we report in full our ground-breaking research into communication within mushroom fruit bodies by chemical signals in the hope that it might stimulate future work in the topic and prompt further advances. We extracted some of the controls of fungal morphogenesis and worked with two components which had recognisably different activities, which we called Fungiflex 1 and Fungiflex 2. Description We believed at the time that we had accessed at least some of the controls of fungal morphogenesis by extracting compounds which caused mushroom stipes to bend at the point of application within hours of treatment. Using standardised bioassays, we made a start on purifying and chemically characterising the extracted substances. We worked with two components which had recognisably different activities in the bioassay. These we called Fungiflex 1 and Fungiflex 2. Our extracts caused (vertical) mushroom stipes to bend towards a drop of the extract within 1 hour of its application to the side of the stipe. This localised growth inhibition was Fungiflex 1 activity. Such stipes grew back to the vertical in about 9 hours, but then started bending in the opposite direction at the point of application of the drop. By 18 hours the stipe had bent to an angle of 90° from the vertical. This localised growth promotion was Fungiflex 2 activity. We got close to understanding the structure of these first fungal hormonal compounds but were unable to secure sufficient funding to take the research any further. Nonetheless, the scientific knowledge we revealed is still important and worthy of publication. Our research has not lost its uniqueness, nor has it been duplicated up to now. In this publication, we report in full the research we accomplished nearly 20 years ago in the hope that it might stimulate future work in the topic and prompt further advances. |
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Buy the PDF-DOWNLOAD of the book (AND PRINT IT YOURSELF) HEREThe advantage of this is that the hyperlinks in the text are live ones, so you can immediately download other publications (if you are connected to the Internet, of course). The downside is that you have to print the thing yourself if you want hard-copy! |
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Greening, J. P. & Moore, D. (1996). Morphometric analysis of cell size patterning involved in gravitropic curvature of the stipe of Coprinus cinereus. Advances in Space Research, 17 (6/7): 83-86. DOI: http://dx.doi.org/10.1016/0273-1177(95)00615-L. CLICK HERE to download the full text.
Greening, J. P., Sánchez, C. & Moore, D. (1997). Coordinated cell elongation alone drives tropic bending in stems of the mushroom fruit body of Coprinus cinereus. Canadian Journal of Botany, 75: 1174-1181. DOI: http://dx.doi.org/10.1139/b97-830. CLICK HERE to download the full text.
Hammad, F., Ji, J., Watling, R. & Moore, D. (1993a). Cell population dynamics in Coprinus cinereus: co-ordination of cell inflation throughout the maturing fruit body. Mycological Research, 97: 269-274. DOI: http://dx.doi.org/10.1016/S0953-7562(09)81119-2. CLICK HERE to download the full text.
Hammad, F., Watling, R. & Moore, D. (1993b). Cell population dynamics in Coprinus cinereus: narrow and inflated hyphae in the basidiome stipe. Mycological Research, 97: 275-282. DOI: http://dx.doi.org/10.1016/S0953-7562(09)81120-9. CLICK HERE to download the full text.
Kher, K., Greening, J. P., Hatton, J. P., Novak Frazer, L. A. & Moore, D. (1992). Kinetics and mechanics of gravitropism in Coprinus cinereus. Mycological Research, 96: 817-824. DOI: http://dx.doi.org/10.1016/S0953-7562(09)81028-9. CLICK HERE to download the full text.
Monzer, J., Haindl, E., Kern V. D. & Dressel, K. (1994). Gravitropism of the basidiomycete Flammulina velutipes: morphological and physiological aspects of the graviresponse. Experimental Mycology, 18: 7-19. DOI: http://dx.doi.org/10.1006/emyc.1994.1002.
Moore, D. (1967a). Four new linkage groups in Coprinus lagopus. Genetical Research, 9: 331-342. DOI: http://dx.doi.org/10.1017/S0016672300010612. CLICK HERE to download the full text.
Moore, D. (1967b). Purine-requiring auxotrophs of Coprinus lagopus (sensu Buller). Journal of General Microbiology, 47: 163-170. DOI: http://dx.doi.org/10.1099/00221287-47-2-163. CLICK HERE to download the full text.
Moore, D. (1969). Sources of carbon and energy used by Coprinus lagopus sensu Buller. Journal of General Microbiology, 58: 49-56. DOI: http://dx.doi.org/10.1099/00221287-58-1-49. CLICK HERE to download the full text.
Moore, D. (1973). Mutants of Coprinus selected for resistance to D-glucosamine and L-sorbose. Genetical Research, 22: 205-209. DOI: http://dx.doi.org/10.1017/ S0016672300013008. CLICK HERE to download the full text.
Moore, D. (1993). Control of pattern and form in mushroom morphogenesis. In Mushroom Biology and Mushroom Products (Proceedings of the First International Conference on Mushroom Biology and Mushroom Products), (S. T. Chang, J. A. Buswell & S.-W. Chiu, eds), pp. 33-39. The Chinese University Press: Hong Kong. CLICK HERE to download the full text.
Moore, D. (2013a). Fungal Biology in the Origin and Emergence of Life. Cambridge, UK: Cambridge University Press. 230 pp. ISBN-10: 1107652774, ISBN-13: 978-1107652774. DOI: http://dx.doi.org/10.1017/CBO9781139524049.
Moore, D. (2013b). Coprinopsis: an autobiography. CreateSpace Independent Publishing Platform: North Charleston, South Carolina, U.S.A. 216 pp. ISBN-10: 1482618974, ISBN-13: 978-1482618976. URL: http://www.amazon.co.uk/Coprinopsis-autobiography-David-Moore/dp/1482618974/ref=tmm_pap_title_0?ie=UTF8&qid=1393606495&sr=1-3.
Moore, D. & Devadatham, M. S. (1975). Distribution of mutant sites in the ftr cistron depends upon the medium used for selection. Molecular and General Genetics, 138: 81-84. DOI: http://dx.doi.org/10.1007/BF00268830. CLICK HERE to download the full text.
Moore, D. & Devadatham, M. S. (1979). Sugar transport in Coprinus cinereus. Biochimica et Biophysica Acta (Biomembranes), 550: 515-526. DOI: http://dx.doi.org/10.1016/0005-2736(79)90153-6. CLICK HERE to download the full text.
Moore, D. Greening, J. P., Hatton, J. P. & Novak Frazer, L. (1994a). Gravitational biology of mushrooms: a flow-chart approach to characterising processes and mechanisms. Microgravity Quarterly, 4: 21-24. CLICK HERE to download the full text.
Moore, D., Hock, B., Greening, J. P., Kern, V. D., Novak Frazer, L. & Monzer, J. (1996). Centenary review. Gravimorphogenesis in agarics. Mycological Research, 100: 257-273. DOI: http://dx.doi.org/10.1016/S0953-7562(96)80152-3. CLICK HERE to download the full text.
Moore, D. & Pukkila, P. J. (1985). Coprinus cinereus: an ideal organism for studies of genetics and developmental biology. Journal of Biological Education, 19: 31-40. DOI: http://dx.doi.org/10.1080/00219266.1985.9654684. CLICK HERE to download the full text.
Moore, D., Robson, G. D. & Trinci, A. P. J. (2011). 21st Century Guidebook to Fungi. Cambridge University Press: Cambridge, U.K. ISBN-13: 978-0521186957. URL: http://www.cambridge.org/9780521186957. VIEW online
Moore, D. & Stewart, G. R. (1971a). Dedikaryotisation of Coprinus lagopus following growth on 2-deoxy-D-glucose. Transactions of the British Mycological Society, 56: 311-313. DOI: http://dx.doi.org/10.1016/S0007-1536(71)80046-3. CLICK HERE to download the full text.
Moore, D. & Stewart, G. R. (1971b). Mutants of Coprinus lagopus selected for resistance to 2-deoxy-D-glucose. Genetical Research, 18: 341-352. DOI: http://dx.doi.org/10.1017/ S0016672300012738. CLICK HERE to download the full text.
Moore, D. & Stewart, G. R. (1972). Effects of 2-deoxy-D-glucose, D-glucosamine, and L-sorbose on the growth of Coprinus lagopus hyphae. Journal of General Microbiology, 71: 333-342. DOI: http://dx.doi.org/10.1099/00221287-71-2-333. CLICK HERE to download the full text.
Novak Frazer, L. (1996). Control of growth and patterning in the fungal fruiting structure. A case for the involvement of hormones. In: Patterns in Fungal Development (ed. S. W. Chiu & D. Moore), pp. 156-181. Cambridge University Press: Cambridge, UK. ISBN-13: 9780521560474, ISBN-10: 0521560470. [CLICK HERE] to visit the Amazon page.
Novak Frazer, L. & Moore, D. (1993). Antagonists and inhibitors of calcium accumulation do not impair gravity perception though they adversely affect the gravitropic responses of Coprinus cinereus stipes. Mycological Research, 97: 1113-1118. DOI: http://dx.doi.org/10.1016/S0953-7562(09)80512-1. CLICK HERE to download the full text.
Novak Frazer, L. & Moore, D. (1996). The role of calcium accumulation and the cytoskeleton in the perception and response of Coprinus cinereus to gravity. Advances in Space Research, 17 (6/7): 87-90. DOI: http://dx.doi.org/10.1016/0273-1177(95)00616-M. CLICK HERE to download the full text.
Pukkila, P. J. & Lu, B. C. (1985). Silver staining of meiotic chromosomes in the fungus, Coprinus cinereus. Chromosoma, 91: 108-112. DOI: http://dx.doi.org/10.1007/BF00294053.
Robert, J. C. & Bret, J. P. (1987). Release of an inhibitor of stipe elongation from illuminated caps of Coprinus congregatus mushrooms. Canadian Journal of Botany 65, 505-508. DOI: http://dx.doi.org/10.1139/b87-063.
Rusmin, S. & Leonard, T. J. (1978). Biochemical induction of fruiting in Schizophyllum. Isolation and preliminary purification of an inducing substance from Agaricus bisporus mushrooms. Plant Physiology 61, 538-543. DOI: http://dx.doi.org/10.1104/pp.61.4.538.
TajAldeen, S. J. & Moore, D. (1982). The ftr cistron of Coprinus cinereus is the structural gene for a multifunctional transport molecule. Current Genetics, 5: 209-213. DOI: http://dx.doi.org/10.1007/BF00391808. CLICK HERE to download the full text.
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